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	Provedor de dados Arq. Bras. Med. Vet. Zootec. (1) BJID (1) Autor Gaspar,Ana Maria Coimbra (1) Hajian,S. (1) Momeni,M. (1) Momtaz,H. (1) Paula,Vanessa Salete de (1) Rahimi,E. (1) Villar,Livia Melo (1) Mais... Palavra-chave HAV (2) RT-PCR (2) Extraction methods (1) Iran (1) Sea food (1) Serum and stool (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Comparison of four extraction methods to detect hepatitis A virus RNA in serum and stool samples BJID Paula,Vanessa Salete de; Villar,Livia Melo; Gaspar,Ana Maria Coimbra. The efficiency of extraction methods for hepatitis A virus (HAV) RNA in clinical samples is of great importance for molecular diagnosis, especially in regions endemic for HAV, such as Brazil. We compared the efficiency of four different extraction techniques in serum and stool samples for the detection of hepatitis A virus by reverse transcription PCR (RT-PCR). We used PCR to analyse serum and stool samples of 12 patients who were referred to the Brazilian Reference Center for Viral Hepatitis (BRCVH) in Rio de Janeiro. The methods tested were Proteinase K, Silica, TRIzol and Guanidine isothiocyanate. Proteinase K extraction was the best method for serum samples; it detected the HAV-RNA in 11 of the 12 samples. The guanidine isothiocyanate method was the... Tipo: Info:eu-repo/semantics/article Palavras-chave: HAV; Extraction methods; Serum and stool; RT-PCR. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200007 Prevalence of hepatitis A virus in sea food in Iran Arq. Bras. Med. Vet. Zootec. Momtaz,H.; Rahimi,E.; Hajian,S.; Momeni,M.. The objective of this study was to determine the prevalence of Hepatitis A Virus (HAV) in sea food samples in the Isfahan and Shahrekord townships in Iran. From September 2010 to April 2011, a total of 300 samples of fresh fish, shrimp, crab and lobster were obtained from randomly selected retail stores in the Isfahan and Shahrekord townships in Iran. The samples were tested for the presence of HAV using a reverse transcriptase- polymerase chain reaction method. Out of the total number of samples examined, 8 (2.7%) were found to be positive for HAV. This virus was detected in 5% and 1.7% of fresh fish and shrimp, respectively. This study shows the importance of sea food as potential sources of HAV infection in people in Iran. Tipo: Info:eu-repo/semantics/article Palavras-chave: HAV; Sea food; RT-PCR; Iran. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400015 Registros recuperados: 2 Primeira ... 1 ... Última

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